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human fibroblast growth factor 2  (R&D Systems)


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    R&D Systems human fibroblast growth factor 2
    Human Fibroblast Growth Factor 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 899 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibroblast+growth+factor+2+fgf2/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pm41978430-44-25-30
    Average 96 stars, based on 899 article reviews
    human fibroblast growth factor 2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: A novel junctional epithelial cell line, mHAT-JE01, derived from incisor epithelial cells.
    Article Snippet: Objectives: Junctional epithelium (JE) connects the tooth surface and gingival epithelium and adheres directly to the tooth enamel.. JE plays an important role as a barrier preventing the invasion of exogenous bacteria and substances.. However, the cellular characteristics of this epithelium have not been adequately described, because no useful in vitro experimental model exists for JE.

    Article Title: CDX2 Promotes Hepatic Specification of hiPSC-derived Endoderm Through the PI3K-Akt-GSK3β Pathway for Improved Therapeutic Efficacy.
    Article Snippet: Hepatobiliary diseases impose a considerable burden on human health.. Approximately 2 million people die each year, accounting for 4% of the global mortality [1, 2].. Conventional treatment methods such as liver transplantation and drug therapy have certain effects.

    Article Title: Selective degradation of FGFR1/2 overcomes antiestrogen resistance in ER+ breast cancer with FGFR1/2 alterations.
    Article Snippet: FGFR1 amplification and FGFR1/2 activating mutations have been associated with antiestrogen resistance in estrogen receptor-positive (ER+) breast cancer.. However, there are no approved FGFR1-targeted therapies for breast cancers harboring these alterations.. In this study, we investigated the selective degradation of FGFR1/2 using the proteolysis-targeting chimera (PROTAC) DGY-09-192 as a novel therapeutic strategy in ER + breast cancers harboring FGFR1/2 somatic alterations.

    Modification:

    Article Title: A novel junctional epithelial cell line, mHAT-JE01, derived from incisor epithelial cells.
    Article Snippet: Objectives: Junctional epithelium (JE) connects the tooth surface and gingival epithelium and adheres directly to the tooth enamel.. JE plays an important role as a barrier preventing the invasion of exogenous bacteria and substances.. However, the cellular characteristics of this epithelium have not been adequately described, because no useful in vitro experimental model exists for JE.

    Generated:

    Article Title: 3D flexible self-folding microelectrode array for organoid electrophysiology analysis
    Article Snippet: Cell Biomaterials 1, 100179, December 23, 2025 9 the open-source Open Ephys GUI software (Open Ephys) with low-pass filtering (1–300 Hz) and high-pass filtering (300– 6,000 Hz) and processed using MATLAB. .. EMLOC gastruloids were generated through a four-stage protocol described previously.38,39 Briefly, in stage 1, 2D hiPSC colonies at approximately 50%–60% confluence were induced for 48 h in induction medium, comprising N2B27 basal medium (1:1 DMEM/F-12 [Thermo Fisher Scientific, cat# 11320033] and Neurobasal Plus [Thermo Fisher Scientific, cat# A3582901] supplemented with 2% [v/v] B-27 [Thermo Fisher Scientific, cat# 17504044], 1% [v/v] N-2 [Thermo Fisher Scientific, cat# 17502048], 1× GlutaMAX [Thermo Fisher Scientific, cat# 35050061], and 1× MEM non-essential amino acids [Thermo Fisher Scientific, cat# 11140050]) with 3 μM 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl] amino]ethyl]amino]-3-pyridinecarbonitrile (CHIR 99021, Tocris Bioscience, cat# 4423) and 40 ng/mL of fibroblast growth factor 2 (FGF2) (R&D Systems, cat# 233-FB), with a full medium exchange at 24 h. In stage 2, the induced colonies were dissociated into a single-cell suspension and transferred to low-adhesion plates in EMLOC gastruloid polarization medium, consisting of N2B27 supplemented with 10 ng/mL FGF2, 2 ng/mL insulinlike growth factor1 (R&D Systems, cat# 291-G1), 2 ng/mL hepatocyte growth factor (R&D Systems, cat# 294-HG), and 50 μM ROCK inhibitor Y-27632 (Tocris Bioscience, cat# 1254, UK), at a density of approximately 2 × 106 cells in 2 mL medium. ..

    Single Cell:

    Article Title: 3D flexible self-folding microelectrode array for organoid electrophysiology analysis
    Article Snippet: Cell Biomaterials 1, 100179, December 23, 2025 9 the open-source Open Ephys GUI software (Open Ephys) with low-pass filtering (1–300 Hz) and high-pass filtering (300– 6,000 Hz) and processed using MATLAB. .. EMLOC gastruloids were generated through a four-stage protocol described previously.38,39 Briefly, in stage 1, 2D hiPSC colonies at approximately 50%–60% confluence were induced for 48 h in induction medium, comprising N2B27 basal medium (1:1 DMEM/F-12 [Thermo Fisher Scientific, cat# 11320033] and Neurobasal Plus [Thermo Fisher Scientific, cat# A3582901] supplemented with 2% [v/v] B-27 [Thermo Fisher Scientific, cat# 17504044], 1% [v/v] N-2 [Thermo Fisher Scientific, cat# 17502048], 1× GlutaMAX [Thermo Fisher Scientific, cat# 35050061], and 1× MEM non-essential amino acids [Thermo Fisher Scientific, cat# 11140050]) with 3 μM 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl] amino]ethyl]amino]-3-pyridinecarbonitrile (CHIR 99021, Tocris Bioscience, cat# 4423) and 40 ng/mL of fibroblast growth factor 2 (FGF2) (R&D Systems, cat# 233-FB), with a full medium exchange at 24 h. In stage 2, the induced colonies were dissociated into a single-cell suspension and transferred to low-adhesion plates in EMLOC gastruloid polarization medium, consisting of N2B27 supplemented with 10 ng/mL FGF2, 2 ng/mL insulinlike growth factor1 (R&D Systems, cat# 291-G1), 2 ng/mL hepatocyte growth factor (R&D Systems, cat# 294-HG), and 50 μM ROCK inhibitor Y-27632 (Tocris Bioscience, cat# 1254, UK), at a density of approximately 2 × 106 cells in 2 mL medium. ..

    Suspension:

    Article Title: 3D flexible self-folding microelectrode array for organoid electrophysiology analysis
    Article Snippet: Cell Biomaterials 1, 100179, December 23, 2025 9 the open-source Open Ephys GUI software (Open Ephys) with low-pass filtering (1–300 Hz) and high-pass filtering (300– 6,000 Hz) and processed using MATLAB. .. EMLOC gastruloids were generated through a four-stage protocol described previously.38,39 Briefly, in stage 1, 2D hiPSC colonies at approximately 50%–60% confluence were induced for 48 h in induction medium, comprising N2B27 basal medium (1:1 DMEM/F-12 [Thermo Fisher Scientific, cat# 11320033] and Neurobasal Plus [Thermo Fisher Scientific, cat# A3582901] supplemented with 2% [v/v] B-27 [Thermo Fisher Scientific, cat# 17504044], 1% [v/v] N-2 [Thermo Fisher Scientific, cat# 17502048], 1× GlutaMAX [Thermo Fisher Scientific, cat# 35050061], and 1× MEM non-essential amino acids [Thermo Fisher Scientific, cat# 11140050]) with 3 μM 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl] amino]ethyl]amino]-3-pyridinecarbonitrile (CHIR 99021, Tocris Bioscience, cat# 4423) and 40 ng/mL of fibroblast growth factor 2 (FGF2) (R&D Systems, cat# 233-FB), with a full medium exchange at 24 h. In stage 2, the induced colonies were dissociated into a single-cell suspension and transferred to low-adhesion plates in EMLOC gastruloid polarization medium, consisting of N2B27 supplemented with 10 ng/mL FGF2, 2 ng/mL insulinlike growth factor1 (R&D Systems, cat# 291-G1), 2 ng/mL hepatocyte growth factor (R&D Systems, cat# 294-HG), and 50 μM ROCK inhibitor Y-27632 (Tocris Bioscience, cat# 1254, UK), at a density of approximately 2 × 106 cells in 2 mL medium. ..

    Derivative Assay:

    Article Title: Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.
    Article Snippet: Accepted Manuscript Title: Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells Author: Hu Yao Qu Zhuang-yin Cao Shi-ying Li Qi Ma Lixiang Robert Krencik Xu Min Liu Yan PII: S0165-0270(16)30023-1 DOI: http://dx.doi.org/doi:10.1016/j.jneumeth.2016.03.017 Reference: NSM 7485 To appear in: Journal of Neuroscience Methods Received date: 13-1-2016 Revised date: 27-3-2016 Accepted date: 28-3-2016 Please cite this article as: Hu Yao, Qu Zhuang-yin, Cao Shi-ying, Li Qi, Ma Lixiang, Krencik Robert, Xu Min, Liu Yan.Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.Journal of Neuroscience Methods http://dx.doi.org/10.1016/j.jneumeth.2016.03.017 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Knock-Out:

    Article Title: Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.
    Article Snippet: Accepted Manuscript Title: Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells Author: Hu Yao Qu Zhuang-yin Cao Shi-ying Li Qi Ma Lixiang Robert Krencik Xu Min Liu Yan PII: S0165-0270(16)30023-1 DOI: http://dx.doi.org/doi:10.1016/j.jneumeth.2016.03.017 Reference: NSM 7485 To appear in: Journal of Neuroscience Methods Received date: 13-1-2016 Revised date: 27-3-2016 Accepted date: 28-3-2016 Please cite this article as: Hu Yao, Qu Zhuang-yin, Cao Shi-ying, Li Qi, Ma Lixiang, Krencik Robert, Xu Min, Liu Yan.Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.Journal of Neuroscience Methods http://dx.doi.org/10.1016/j.jneumeth.2016.03.017 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Recombinant:

    Article Title: Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.
    Article Snippet: Accepted Manuscript Title: Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells Author: Hu Yao Qu Zhuang-yin Cao Shi-ying Li Qi Ma Lixiang Robert Krencik Xu Min Liu Yan PII: S0165-0270(16)30023-1 DOI: http://dx.doi.org/doi:10.1016/j.jneumeth.2016.03.017 Reference: NSM 7485 To appear in: Journal of Neuroscience Methods Received date: 13-1-2016 Revised date: 27-3-2016 Accepted date: 28-3-2016 Please cite this article as: Hu Yao, Qu Zhuang-yin, Cao Shi-ying, Li Qi, Ma Lixiang, Krencik Robert, Xu Min, Liu Yan.Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells.Journal of Neuroscience Methods http://dx.doi.org/10.1016/j.jneumeth.2016.03.017 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.



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    R&D Systems basic fibroblast growth factor 2
    Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; <t>FGF2.</t> Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.
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    Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; <t>FGF2.</t> Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.
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    Image Search Results


    Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; FGF2. Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.

    Journal: Military Medical Research

    Article Title: Engineering of functional auditory neurons from human induced pluripotent stem cells

    doi: 10.1016/j.mmr.2026.100008

    Figure Lengend Snippet: Stepwise differentiation of hiPSCs into human ONPs. a Overview and timeline of human SGN-like neuron differentiation protocol from hiPSCs and matching bright-field images. Scale bar=100 μm. b Comparison of cells at D25 and D63 following seeding at different cell densities (25,000 or 60,000 cells/cm 2 ) on D11 after sorting with NGFR beads. Scale bar=100 μm. c Immunostaining for NGFR + NESTIN + precursors of PPE and neural crest cells at D12. Scale bar=100 μm. d - f Immunostained D25 cells. Antibodies highlight neuroblast cells (NEUROD1 + ) and otic lineage cells (PAX2 + , PAX8 + , and SOX2 + ), but no expression of neuronal markers (TUBB3) at this stage. Scale bar=100 μm. g Bar graphs showing the average proportion (%) of positive cells on D25 cells stained with NEUROD1, PAX2, PAX8, SOX2, and TUBB3. Error bars represent SD. hiPSCs. Human induced pluripotent stem cells; ONPs. Otic neural progenitors; BMP4. Bone morphogenetic protein 4; LDN. LDN-193189; IWP2. Inhibitor of Wnt production-2; FGF2. Basic fibroblast growth factor 2; NGFR. Nerve growth factor receptor; Wnt3. Wnt family member 3; IGF-1. Insulin-like growth factor 1; SHH. Sonic hedgehog; RA. Retinoic acid; EGF. Epidermal growth factor; NEUROD1. Neurogenic differentiation 1; cAMP. Cyclic adenosine monophosphate; NT3. Neurotrophin 3; BDNF. Brain-derived neurotrophic factor; TUBB3. Tubulin beta 3 class III; PRPH. Peripheral neuronal marker peripherin; MPZ. Myelin protein zero; PPE. Pre-placodal ectoderm; SGC. Satellite glial cells; D. Day; SD. Standard deviation; GFAP. Glial fibrillary acidic protein; PAX2. Paired box gene 2; SOX2. SRY-box transcription factor 2; DAPI. 4′;6-diamidino-2-phenylindole.

    Article Snippet: On D3, the medium was replaced with a CDM containing 10 ng/ml bone morphogenetic protein 4 (BMP4; Cat# 314-BP, R&D Systems, USA), 1 μmol/L SB-431542 (Cat# 1614, TOCRIS, UK), and 10 ng/ml basic fibroblast growth factor 2 (FGF2, Cat# 233-FB, R&D Systems, USA).

    Techniques: Comparison, Immunostaining, Expressing, Staining, Derivative Assay, Marker, Standard Deviation

    Origin of human SGN-like neurons revealed by scRNA-seq. a Schematic illustration of the developmental origins of auditory neurons in the human inner ear in vivo . b UMAP plots of SK8-A D25 cells, color-coded as in  a. c Feature plots showing key gene markers for the classification of cell subtypes and determination of neuronal origins. d Re-clustering of isolated otic neural primordium cells from  b, separating into three populations: EMT, delaminating neuroblasts, and neurogenesis. e Heatmap showing the top 100 differentially expressed genes across the three distinctive clusters: EMT, NB, and NG. The X and Y axes represent differentially expressed genes and single cells, respectively. f Violin plots displaying marker gene expression in a log-transformed scale among the three different clusters of otic neural primordium. The number in the upper right corner represents log-normalized gene expression (log1p normalized UMI counts) per cell. g Feature plot visualizing expression of SGN and VGN markers in the otic neural primordium cluster, based on scRNA-seq data from previous studies  . Numbers in the lower left corners indicate the percentage of cells expressing each marker. h Dot plots illustrating minimal expression of hindbrain, GG, and EPI markers, and high expression of otic neuroblast and neuronal markers. To support data sharing, visualization, and analysis, the processed scRNA-seq dataset for  is available on the gEAR portal ( https://umgear.org/p?l=sk8aNeuron ). SGN. Spiral ganglion neuron; scRNA-seq. Single-cell RNA sequencing; UMAP. Uniform manifold approximation and projection; EMT. Epithelial-mesenchymal transition; NB. Neuroblasts; NG. Neurogenesis; VGN. Vestibular ganglion neurons; GG. Geniculate ganglion; EPI. Epibranchial placode; PLP1. Proteolipid protein 1; TWIST1. Twist family bHLH transcription factor 1; TOP2A. DNA topoisomerase II alpha; MEUROD1. Neuronal differentiation 1; SOX2. SRY-box transcription factor 2; WNT4. Wnt family member 4; FGF8. Fibroblast growth factor 8; DCX. Doublecortin; MEIS2. Meis homeobox 2; PROX1. Prospero homeobox 1; TLX3. T cell leukemia homeobox 3; SALL3. Spalt like transcription factor 3; PVALB. Parvalbumin; CNC. Cranial neural crest; ZBTB16. Zinc finger and BTB domain containing 16; PAX6. Paired box 6; HOXA2. Homeobox A2; FOXI1. Forkhead box I1; PHOX2A. Paired mesoderm homeobox protein 2A; FOXI2. Forkhead box I2; NEUROG1. Neurogenin 1; INSM1. Insulinoma-associated 1; HE56. Hes family BHLH transcription factor 6; MAP2. Microtubule-associated protein 2; TUBB3. Tubulin beta 3 class III.

    Journal: Military Medical Research

    Article Title: Engineering of functional auditory neurons from human induced pluripotent stem cells

    doi: 10.1016/j.mmr.2026.100008

    Figure Lengend Snippet: Origin of human SGN-like neurons revealed by scRNA-seq. a Schematic illustration of the developmental origins of auditory neurons in the human inner ear in vivo . b UMAP plots of SK8-A D25 cells, color-coded as in a. c Feature plots showing key gene markers for the classification of cell subtypes and determination of neuronal origins. d Re-clustering of isolated otic neural primordium cells from b, separating into three populations: EMT, delaminating neuroblasts, and neurogenesis. e Heatmap showing the top 100 differentially expressed genes across the three distinctive clusters: EMT, NB, and NG. The X and Y axes represent differentially expressed genes and single cells, respectively. f Violin plots displaying marker gene expression in a log-transformed scale among the three different clusters of otic neural primordium. The number in the upper right corner represents log-normalized gene expression (log1p normalized UMI counts) per cell. g Feature plot visualizing expression of SGN and VGN markers in the otic neural primordium cluster, based on scRNA-seq data from previous studies . Numbers in the lower left corners indicate the percentage of cells expressing each marker. h Dot plots illustrating minimal expression of hindbrain, GG, and EPI markers, and high expression of otic neuroblast and neuronal markers. To support data sharing, visualization, and analysis, the processed scRNA-seq dataset for is available on the gEAR portal ( https://umgear.org/p?l=sk8aNeuron ). SGN. Spiral ganglion neuron; scRNA-seq. Single-cell RNA sequencing; UMAP. Uniform manifold approximation and projection; EMT. Epithelial-mesenchymal transition; NB. Neuroblasts; NG. Neurogenesis; VGN. Vestibular ganglion neurons; GG. Geniculate ganglion; EPI. Epibranchial placode; PLP1. Proteolipid protein 1; TWIST1. Twist family bHLH transcription factor 1; TOP2A. DNA topoisomerase II alpha; MEUROD1. Neuronal differentiation 1; SOX2. SRY-box transcription factor 2; WNT4. Wnt family member 4; FGF8. Fibroblast growth factor 8; DCX. Doublecortin; MEIS2. Meis homeobox 2; PROX1. Prospero homeobox 1; TLX3. T cell leukemia homeobox 3; SALL3. Spalt like transcription factor 3; PVALB. Parvalbumin; CNC. Cranial neural crest; ZBTB16. Zinc finger and BTB domain containing 16; PAX6. Paired box 6; HOXA2. Homeobox A2; FOXI1. Forkhead box I1; PHOX2A. Paired mesoderm homeobox protein 2A; FOXI2. Forkhead box I2; NEUROG1. Neurogenin 1; INSM1. Insulinoma-associated 1; HE56. Hes family BHLH transcription factor 6; MAP2. Microtubule-associated protein 2; TUBB3. Tubulin beta 3 class III.

    Article Snippet: On D3, the medium was replaced with a CDM containing 10 ng/ml bone morphogenetic protein 4 (BMP4; Cat# 314-BP, R&D Systems, USA), 1 μmol/L SB-431542 (Cat# 1614, TOCRIS, UK), and 10 ng/ml basic fibroblast growth factor 2 (FGF2, Cat# 233-FB, R&D Systems, USA).

    Techniques: In Vivo, Isolation, Marker, Gene Expression, Transformation Assay, Expressing, Single Cell, RNA Sequencing